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Image Search Results
Journal: bioRxiv
Article Title: The cold-inducible RNA-binding protein RBM3 stabilises viral mRNA at the cooler temperatures of the upper respiratory tract
doi: 10.1101/2024.07.25.605104
Figure Lengend Snippet: (A) Cold-responsiveness of a panel of cell lines and primary nasal epithelial cells (PNECs) as determined by the induction of RBM3 expression by qPCR over 48 h and normalised to 37°C levels. (B) Generation of a lentiviral RBM3 overexpression (RBM3+) A549 cell line alongside a control empty vector line (EMP). (C) Titres of WSN at 72 hpi after an MOI 0.01 infection in EMP or RBM3+ A549 cells. (D) RBPmap predicted RBM3 binding motifs for each WSN positive strand segment, separated by predicted confidence of each motif. (E) Western blot showing input and pulldown samples for CLIP-qPCR, blotting for Actin and overexpressed RBM3-Flag. (F) qPCR quantification of the RNA extracted from CLIP samples demonstrated a significant increase in viral NP mRNA levels with minimal differences in HA or Actin transcripts, when comparing RBM3 to no antibody control pulldowns.
Article Snippet: Cells were submerged in modified
Techniques: Expressing, Over Expression, Control, Plasmid Preparation, Infection, Binding Assay, Western Blot
Journal: Brain : a journal of neurology
Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.
doi: 10.1093/brain/awr338
Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.
Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in
Techniques: In Vitro, Control